Composite

Part:BBa_K2128204:Design

Designed by: Alejandro Schmieder, Alejandro Schmieder, Whitney Wong, David Sanderson   Group: iGEM16_Genspace   (2016-10-19)


HDLEA1 with strong promoter/RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have attempted to maximize expression of HDLEA1 through use of a strong constitutive promoter (J23100) and strong ribosome binding site (B0034).


Source

The coding sequence for HDLEA1 was taken from the genome of Ramazzottius varieornatus and optimized by the IDT Codon Optimization Tool for E. Coli K12

References